【摘要1目的 比较微量取样与一次取样的优劣。方法36只家兔分为4组(每组9只)，于死后12 h取样：i组于一次取
出所有vh；ii组微量取样50 l，连续取15次；iii组左眼一次取样。右眼微量重复取样
。iv组双眼第1次微量取样．剩余一次
取出。检测ca2+cl 、l(+、na 和p等含量，数据统计分析。结果 iii组左右眼、(iv)组先后两次取样配对t检验无差异(p>0
．05)，
各组微量取样样本的变异系数、双眼差较小。结论 微量取样的操作影响较小、稳定性和重复性好
． 更适合法医学研究和应
用。
【关键词】尸体化学；玻璃体液：取样方法：比较
【中图分类号】d919．1
【文献标识码】a
【文章编号】1007—9297(2006)03—0208—03
a comparison study of vitreous humor micro-sam pling and wholly sam pling on rabbits． zha ng yu—zhuj，w a ng lan2．3．
liu l ，(department offorensic me dicine，ton#ji medical college ofhuazhong university ofscience arm techno ，w han
430030,chian)
[abstract] ive to make a comparative study of wholly sampling and micro-sampling of vitreous humor(vh)．
methods
【作者简介】战玉祝(1968一)，男，汉族，山东莒南县人，医学学士，副主任法医师
， 研究方向：临床法医学中的视觉、听觉的电生理研究以
及法医病理的死亡时间研究；tel~+86—531—86323203；e-mail：james6803@21cn．com
【通信作者1刘良(1960一)，男，汉族，山东沂水人，教授，博士生导师，主要从事法医病理学教学
、研究及检案。
法律与医学杂志2006年第13卷(第3期)
thirty—six rabbits were divided into four groups，each of which contains nine．all were sampled at 12h postmortem．group 1 were
wholly extracted． group i1 were subjected to multiple sampling． of group iii， all left eyes were wholly sampled and all right eyes
were micro-sampled．all eyes of group iv were micro—sampled at first and all the left were wholy sampled．concentrations of calcium，
chloride，potassium，sodium and phosphorus were detected and statistically analyzed．results th ere are no significan t difference
between wholly sampling and micro-sampling of the pair-matched t—test of between-eyes in group iii and the twice sanipiing
in group iv．of micro—sam pling，the variations of the both-eyes and coeficient of variation were smaller than those of whony
sam pling． conclusion th e multi-micro-sam pling operation makes much less distort on vh， and is more stab le and repeatable，
which is promising for forensic vh study an d examination．
【key words l postmortem chemistry；vitreous humog sampling methodology；comparison study
取样方法对玻璃体液(vitreous humor，vh)研究
有重要意义，[11传统采用一次取样vh，近年有研究使
用了微量取样。迄今未见两取样方法比较研究，本实
验通过一次取样与微量取样的比较． 旨在为vh研究
选择取样方法。
材料与方法
一
、实验动物及分组取材
健康家兔36只，雌雄混用，体重1．9～2．5 ，由华
中科技大学同济医学院实验动物中心提供。耳缘静脉
空气栓塞处死，置于4～7℃恒温箱，随机分为4组(每
组9只)，于死后12h取样：i组根据coe推荐的方法，
用20g针头5 iill注射器自外眦、角膜缘外约2 mm
处缓慢进针，瞳孔观察针头位于中央时缓慢抽出所有
vh，摇匀后移液50 l与去离子水1：4稀释，编号入
ep管、-25℃冻存。ii组微量重复取样：用20 g留置
针，入针后固定针